Practical Molecular Biology
Application of modern methods to the quantitative generation of active proteins using prokaryotic and eukaryotic expression systems. Studies the identification and generation of gene constructs corresponding to potentially soluble (and active) protein subunits, and explores the optimization of their expression and purification. The use of different organisms to overcome problems of protein folding and glycosylation will be discussed.
Graduate course in the Biochemistry and Microbiology program administered by the Faculty of Graduate Studies.