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Cryo-EM day

Friday was our cryo-EM day. The set up itself is pretty complex, and it took a while to get things up and running. The challenge revolves around getting everything in alignment, since the accuracy of the machine is so high. By the end of the day we were on our way with the collection running until Monday. You can see the tired crew in the photo. Proteins are distributed in a gold grid that has, in this case, a very thin gold foil with holes in it. There are several hundred squares on the grid, and several hundred holes in each grid square. We set up to count more than a thousand holes. A sample image is displayed on this page. The dark clusters are protein complexes. You can see that there is very little information in each picture. In the next step, the data will be compiled from all of the images and processed. Hopefully, at the end, a beautiful high resolution image will emerge!