AEROLYSIN RECEPTORS AND APPLICATIONS TO HUMAN DISEASE

Brodsky, R. A., Mukhina, G. L., Li, S., Nelson, K. L., Chiurazzi, P. L., Buckley, J. T., and Borowitz, M. J. (2000) Improved detection of paroxysmal nocturnal hemoglobinuria using fluorescent aerolysin. Amer. J. Clin. Pathol. 114:459-66.

Mackenzie, C. R., Hirama, T., and Buckley, J. T. (1999) Analysis of Receptor Binding by the Channel Forming Toxin Aerolysin Using Surface Plasmon Resonance. J. Biol. Chem. 274: 22604-22609.

Nguyen, D. H., Buckley, J. T., and Hildreth, J. E. K. (1999) The channel-forming toxin aerolysin neutralizes human immunodeficiency virus type 1. Mol. Microbiol. 33: 659-666.

Brodsky, R. A., Mukhina, G., Nelson, K. L., Lawrence, T. S., Jones, R. J., and Buckley, J. T. (1999) Resistance of paroxysmal nocturnal hemoglobinuria cells to the glycosylphosphatidylinositol-binding toxin aerolysin. Blood 93: 1749-1756.

Diep, D. B., Nelson, K. L., Raja, S. M., Pleshak, E. N., and Buckley, J. T. (1998) Glycosylphosphatidylinositol anchors of membrane glycoproteins are binding determinants for the channel-forming toxin aerolysin. J. Biol. Chem. 273: 2355-2360

STRUCTURE-FUNCTION STUDIES ON CHANNEL-FORMING PROTEINS

Alonso, A., Goñi, F, and Buckley J. T. (2000) Lipids favoring inverted phase favor aerolysin insertion into liposome bilayers. Biochemistry 39, 14019-14024.

Nelson. K. L., and Buckley, J. T. (2000) Channel Formation by the GPI-Anchored Protein Binding Toxin Aerolysin is not Promoted by Lipid Rafts. J. Biol. Chem. 275: 19839-19843.

Rossjohn, J., Raja, S. M., Nelson, K. L., Feil, S. C., van der Goot, F. G., Parker, M. W., and Buckley, J. T. (1998) Movement of a loop in domain 3 of aerolysin is required for channel formation. Biochemistry 37: 741-746.

Rossjohn, J., Feil, J. C., Mckinstry, W. J., Tsernoglou, D, van der Goot, G., Buckley, J. T., and Parker, M. W. (1998) Aerolysin - A paradigm for membrane insertion of beta-sheet protein toxins? J. Struct. Biol. 121: 92-100.

TYPE II SECRETION

Diep, D. B., Nelson, K. L., Lawrence, T. L., Sellman, B. R., Tweten, R. K, and Buckley, J. T. (1999) Expression and properties of an aerolysin-Clostridium septicum alpha toxin hybrid protein. Molec. Microbiol 31: 785-794

Diep, D. B., Lawrence, T. S., Ausio, J., Howard, S. P., and Buckley, J. T. (1998) Secretion and properties of the large and small lobes of the channel-forming toxin aerolysin. Molec. Microbiol. 30: 341-352.