Chem 560 Research Tools and Special Topics
NMR characterization of dynamic systems
Instructor: Fraser Hof
Content: This module will teach
practical methods for determination of thermodynamic and
kinetic parameters of assembling systems. The primary
focus will be on using 1D- and 2D-NMR techniques to
determine association constants and other characteristics
of reversibly assembling systems, and will include a
discussion of the math that is required to understand
chemical shift in dynamic systems, equilibrium models, the
1:1 binding isotherm, and graphical methods for data
analysis. Other topics covered might include the use of
Isothermal titration calorimetry (ITC), UV-Vis,
fluorescence, and potentiometry.
Delivery (all classes in Petch 043e -
the Centre for Biomedical Research Conference Room):
Wed. Nov.
6, 9:00 am - 11:30 am
Wed. Nov.
13, 9:00 am - 11:30 am
Thurs. Nov
28. 2:00 pm - 4:30 pm
Thurs. Dec.
5, 1:00 pm - 4:00 pm.
Final exam:
Dec 17, 9:00 am - 12:00 pm, to be held in Ell 305.
Assessment: An in-class presentation, a written assignment, and a Final exam. See the syllabus for details.
Syllabus and
outline of topics
Slides
Problem set - answers
Final Exam 2010 - answers
Final Exam 2012 - answers
Student slides
Dynamic Light Scattering - PaulLine shape analysis - Alok (notes)
DOSY - Natasha
FRET - Vahid
ITC - Wei