Standard Operating Procedures

Retraining videos

New workers in the group receive all sorts of training, and it can sometimes be a bit overwhelming. The videos on this page (YouTube channel) are intended to give you a quick refresher on how to do something - they're basically a video version of a standard operating procedure. They are NOT a substitute for the initial hands-on training, and you should NOT attempt to perform these operations unless you have received that training first. If you want to learn how to do something here, contact Scott.

Handling air- and moisture-sensitive materials

• Using a glovebox
• How to perform a vacuum sublimation
• How to change the gloves on a glovebox
• Changing a nitrogen cylinder
• Freeze, pump, thaw solvents on the Schlenk line
• Solvent vacuum distillation
• Degassing solvent on the Schlenk line
• How to use the solvent purification system
• How to remove a sample from the glovebox
• How to regenerate the small glovebox
• Regenerate the large glovebox
• Cannula transfer

In the lab

• Procedures in case of fire
• Safe handling of needles
• Ordering with Quartzy
• How to use the rotovap
• How to change a base bath
• How to refill a solvent on the SPS
• Shutting down the lab at the end of the day

Software

• SOAPy (Lars' data binning python script). Files at https://github.com/larsyunker/mass-spec-python-tools
• PythoMS installation for Windows
• Convert Raw file to mzML format
• Isotope pattern overlay
• Pyrsir
• Video render script, Mac version
• Video render script, Windows version
• AutoHotKey
• COPASI parameter estimation

Micromass QTof micro

• Calibrating the QTof micro
• Changing the MS capillary part 1
• Changing the MS capillary part 2
• Ballast the MS vacuum pump

Waters TQD

• TQD and photodiode array detector
• TQD editing MS files for SIR, MRM
• TQD intellistart menu, instrument resolution and tuning
• SIR for metal complexes
• TQD scope window functions
• TQD real time instrument parameters
• Shut down the TQD
• TQD cleanup
• TQD solvent aggregation

Bruker ALPHA FTIR

• How to start up the Bruker ALPHA
• How to take an IR measurement with the Bruker ALPHA
• Set up for continuous IR readings
• Reaction monitoring Bruker ALPHA
• Changing the pump valves Bruker ALPHA

Archived videos (no longer relevant)